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Author Topic: micropropigation preparation workspace  (Read 33567 times)


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Re: micropropigation preparation workspace
« Reply #90 on: June 25, 2021, 08:34:52 pm »

just had one areole on each of mine.
but a bigger piece would grow faster.

i can't remember exactly, but it was pretty bad..
it was something like 40-50% became contamed,
and 30-40% no contams, but dead from sterilizing.
then i threw away some that might have survived.
so i'm left with three jars that are growing good.

they have root and pups, so i'm planning to
transfer to sterile sandy soil sometime soon.
we all come from the garden and to it we shall return


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Re: micropropigation preparation workspace
« Reply #91 on: June 25, 2021, 09:02:06 pm »

Yea mine is terrible. I've got 70% contam so far from like 10 jars.

What was the condition of the plants before you took cuts?

I cut some pieces from the base of a T. Pachanoi that has been in the process of rooting for 3 months. This is likely my issue because there is already some very slight rot going on in the base. It has etiolated terribly so I might just cut the tip and give it a go as it's fresh and hopefully somewhat cleaner growth. I haven't got much stock to experiment with so I'm just going with this for now.

The guy on FB with lots of Cacti TC experience is almost always using seed. Seems the better route to go as sterilization is simpler/easier.

Please post some pics once you get those in the soil!  :)
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