i think the antibiotics we use most in the lab are benomyl, refampicin, kanamycin, & streptomycin. i'll have to check some notes, but pretty sure these can all be used in plant tissue culturing. but we almost always use two or more antibiotics, i think it helps limit the amount of microbial resistance that may develop over time. we dont want to breed antibiotic resistant bacteria and such...
if you are working with healthy plants and clean the exterior surfaces well, you might can get away without any antibiotics
as to which media to use, i would google scholar search some primary lit and try to find folks who cloned related plants to what you want to work with, read through the materials & methods sections, and see what was included in the media used. that stuff gets a bit dense sometimes, but it will probably tell you why the particular media was used as well, and then maybe you can adjust according to your needs. of course this wont be of much help for really obscure plants, but worth checking out, could save a lot of time and resources and headache!
i think the hormone levels are really important, and different groups of plants are likely to prefer different ratios.
rooting hormone is a form of auxin- if used too generously on easy-to-root plants such as succulents or vines, it tends to be counteractive, inhibiting root development. these plants root easy because their endogenous auxin levels are "perfect", so to say. whereas, hard to root plants really benefit from small amounts of rooting hormone, and some can be almost impossible to root without hormonal assistance.
the same is going to happen in your media- some plants will appreciate a generous amount of auxin, others will die at these concentrations.
same goes for cytokinins
and then these groups of hormones play off each other, so relative ratios to each other are also important, and preferred ratios likely differ between species.
i think growing two different species or even genera of for example Solanaceae family will probably both work well in similar media,
but something like Diplopterys from the Rubiaceae family in the Asterids, might require a totally different media than Acacia from the Fabaceae family in the Rosids... i have no idea really, but i would not be surprised.
really, if you have the time, space, and patience, itd be awesome if you set this up like an experiment, and created multiple sets of cloning media, with different ratios of antibiotics and hormones, and record how the clones of a single plant type perform. then repeat with more plants. we all could learn a lot from that work.
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Topic: micropropagation preparation workspace (Read 77844 times)