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Author Topic: DIY DNA GMO  (Read 8173 times)

ONandONandON

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DIY DNA GMO
« on: January 20, 2017, 05:44:55 PM »
Ok well glowingplant.com is slacking.... anyone know when they'll be released? >(never)<

The subject has come up a couple of times, but nothing much about doing this ourselves..

http://makezine.com/2013/05/16/diy-synthetic-biology-making-your-own-glowing-plants/


One issue is obtaining the glowing DNA, here is a simple DNA extraction video...

maybe it could be improved on so maybe we can extract fireflies or something. (edit:i would never crush fireflies)

Here is a brief discription of the "MAKE DNA transfer tek"... ..
First isolate agrobacterium  (A. tumefaciens or  A. rhizogenes) and grow extra cultures for experimenting..
Then place the DNA together with the agrobacteria in small tubes, and freeze shock it (break open cells) and shake it...
and regrow the agrobacteria on plates and look for signs of the new glowing DNA, isolate and grow the glowing strain.
Next grow the glowing agrobacteria in water/nutrients, and simulataneously have plants going into seed.
Dip developing seed pods into the glowing agrobacterium soup, wich should infect the developing seeds with new DNA.

Also there are several types of bioluminescent bacteria, one example is Aliivibrio fischeri found living on squid skin...
this company, http://www.glowee.eu/ has transfered the glowing squid bacteria DNA to more easily grown bacteria.

Well could be done.. anyway if anyone has extra glowing genetically modified agrobacterium, i'd be interested.  ;)
« Last Edit: January 20, 2017, 05:46:19 PM by ONandONandON »
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GrowerAndaShower

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Re: DIY DNA GMO
« Reply #1 on: January 23, 2017, 06:37:18 PM »
I looked into genetic modification a couple years ago, and IIRC there were kits available with bacteria and dna for GFP.
Might be worth looking into Luciferase genetic kits as well.

Found a link for firefly luciferase plasmid,  however "This material is available to academics and nonprofits only."
https://www.addgene.org/18964/
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BubbleCat

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Re: DIY DNA GMO
« Reply #2 on: January 23, 2017, 07:38:51 PM »
Interesting ghetto approach on introducing dna into cells, never thought of it like that.
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Mangrove

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Re: DIY DNA GMO
« Reply #3 on: June 22, 2017, 05:52:12 PM »
http://www.the-odin.com/diy-crispr-kit/

Enjoy these fireworks, kiddos! If you happen to cook-up a strain of E. Coli resistant to all major classes of antibiotics, please let me know. ASAP.
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