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[modern]

So I'll be sowing these seeds tomorrow after 24 hour soak in water.

I setup 3 soaks with 100ml water and 20ml H2O2 each.
The first is control followed by 200-250ppm GA3 and 500ppm GA3. They are actually a bit lower ppm since I calculated the mg of GA3 based on 100mL of water.

4 Likes prasada, Shamichael, Pollinator and Greenmystery like this.

[modern]

I ended up only leaving for 17h in GA3 and 1h in normal water soak. Planted them with the wing pointing up maybe 2cm deep. sphagnum moss was mixed with the top layer of the soil since I didn't want to use pure moss for the seeds.

I'll update in around a month as soon as I notice the first sprouts.

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[modern]

So at 1 month still no sprouts. HOWEVER I had a surprise germination of a psychotria viridis. I used some soil that had seeds in it and it sprouted after a month so conditions are good.

1 Like Pollinator likes this.

[modern]

The seed that germinated isn't psychotria viridis like I thought IDK what it is but I'll let it grow a while.

Today on the 500ppm soaked seeds I spotted a root nub coming from a seed head so I finally got my first germination and hopefully soon a sprout. The other seeds I can't tell since I don't wanna uproot and potentially damage it.

This paper I found after I started my experiment... they did a 500 ppm ga3 soak 24 hours but found it better to apply 1ml of that solution directly in the substrate with the seed since they believe that the penetration/uptake of the seed is too low with the Banisteriopsis they were studying. For those interested in experimenting with ga3 this is a good starting point.
https://www.scielo.br/j/bjb/a/frs6ZYMQ4B4bw6GVPn3qJPm/?lang=en

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[Shamichael]

Thanks for the link and fingers 🤞🏼.