Share The Seeds
Botany and Research => Plant Science => Topic started by: chronic on July 26, 2017, 12:51:02 PM
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Screening of Hallucinogenic Compounds and Genomic Characterisation of 40 Anatolian Salvia Species.
Author information
1 TUBITAK National Metrology Institute (UME), Reference Materials Laboratory, 41400, Kocaeli, Turkey.
2 Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, 34469, Istanbul, Turkey.
3 Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), 41400, Kocaeli, Turkey.
4 Organic Chemistry Laboratory, TUBITAK National Metrology Institute (UME), 41400, Kocaeli, Turkey.
5 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bezmialem Vakıf University, 34093, Istanbul, Turkey.
Abstract
INTRODUCTION:
Salvia, an important and widely available member of Lamiaceae family. Although comparative analysis on secondary metabolites in several Salvia species from Turkey has been reported, their hallucinogenic chemicals have not been screened thoroughly.
OBJECTIVE:
This study provides LC-MS/MS analysis of 40 Salvia species for screening their psychoactive constituents of salvinorin A and salvinorin B. 5S-rRNA gene non-coding region of Salvia plants was sequenced, aligned and compared with that sequence of Salvia divinorum plant.
METHODOLOGY:
Targeted molecules of salvinorin A and salvinorin B were quantified, using LC-MS/MS, from all aerial parts of 40 Salvia species, collected from different parts of Turkey. Regions of 5S-rRNA gene from different species were amplified by polymerase chain reaction and DNA sequences were aligned with Salvia divinorum DNA sequences.
RESULTS:
Very few of the Salvia species (S. recognita, S. cryptantha and S. glutinosa) contained relatively high levels of salvinorin A (212.86 ± 20.46 μg/g, 51.50 ± 4.95 μg/g and 38.92 ± 3.74 μg/g, respectively). Salvinorin B was also found in Salvia species of S. potentillifolia, S. adenocaulon and S. cryptantha as 2351.99 ± 232.22 μg/g, 768.78 ± 75.90 μg/g and 402.24 ± 39.71 μg/g, respectively. The sequences of 5S-rRNA gene of 40 different Salvia species were presented and it was found that none of the Salvia species in Turkey had similar DNA sequence to Salvia divinorum plant.
CONCLUSION:
This is the first report of screening 40 Salvia species in Turkey according to their psychoactive constituents, salvinorin A and salvinorin B and their genomic structures. It is possible that some of these Salvia species may exhibit some psycho activity. Thus, they need to be screened further. Copyright © 2017 John Wiley & Sons, Ltd.
Copyright © 2017 John Wiley & Sons, Ltd.
https://www.ncbi.nlm.nih.gov/pubmed/28722248 (https://www.ncbi.nlm.nih.gov/pubmed/28722248)
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Super interesting! If anyone wants a copy, you are welcome to pm me
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that's amazing great find 8)
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If not downright earth shaking! Good to know our Salvinorin is more widely expressed than just in one single, special species (Much as we may love her.)
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That's pretty cool, and recognita looks like it's available through a few sites with a google search.
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Some of the others are available as well.
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Probably true, however I only googled potentillifolia and recognata(because they were the first listed and most potent, and I was on my way out the door). Potentifillia is mentioned on a few sites selling salvias, but I have not found one for sale(or it's seeds) yet.
Really did not look hard though, maybe 10 mins between both species.
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Are they endemic to the Fertile Crescent and other areas of the middle-east, as well? I have a hunch that this somehow impregnated the mid-east with the concept of organised religion...
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I'm not in a position to buy or help with a co-op but maybe someone else can help spread these seeds within the community.
http://www.kpr-eshop.eu/pt/perennials/?slist_page=0&slist_letter=S
They carry two of the species listed Salvia cryptantha which has both a and b and glutinosa which has just a. This is an interesting find and hope to grow some when I have a chance
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I have still found a revealing text to the potent salvinorin-containing sage species.
Of the 40 Salvia tested, three species contain a larger amount of salvinorin A, so these plants could actually be used as a psychedelic / exhilarating & healing remedy.
The three species are Salvia recognita, Salvia cryptantha and Salvia glutinosa.
The tested content of S. recognita was 212.86 ± 20.46 μg / g, the content of S. cryptantha was 51.50 ± 4.95 μg / g, and that of S. glutinosa was 38.92 ± 3.74 μg / g.
For comparison, Salvia Divinorum contains about 100-500 μg / g salvinorin A.
Salvia glutinosa is also common in the rest of Europe and in West Asia!
Sticky sage (Salvia glutinosa)
Also in Germany, Austria and Switzerland these plants can rarely be found. It is also called Sticky Sage or Yellow Sage.
The newly discovered psychoactive sage species are therefore somewhat less potent than Salvia Divinorum, but Salvia recognita is still so potent that it could potentially be used as a drug.
Source: http://www.magischepflanzen.de/neue-psychoaktive-salvia-arten/
For those who do not understand German please use google translator.
Salvia glutinosa and S. recognita seeds are easy to order in Europe.
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Anybody have any info about the cold-hardness of the new species ? ?
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Salvia Recognita:
Family: Lamiaceae
Hardiness: To Zone 5
Mature Size: 3'
Exposure: Sun/Pt Sun
Bloom Time: June-Jul
Moisture Needs: Dry to Average
Deer Resistant: Yes
Origin: Turkey
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I so knew I'm right on setting out to collect possibly ALL salviae !
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S. recognita 2n = 16
S. potentillaefolia 2n = 16
They might form a hybrid ;D
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Thanks Auxin for the important reference. Maybe this can be important in future.
I so knew I'm right on setting out to collect possibly ALL salviae !
The Salvia species are really very interesting. Many have pain suppressing properties. Especially when you make extracts from them. Recently, the ethanolic extract of aerial part of salvia aethiopis exhibited in tests moderate to weak activity towards delta and kappa opioid receptors.
Salvia guaranitica contains Cirsiliol which has soothing and hypnotic properties in pure form like benzodiazepines.
Other species are no less interesting. It comes a lot more in future.
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Since someone hmu on easy S G and S R seeds I have checked pricing, as suggested stuff is ridicolous.
Can have:
190 seeds of S G for 1,50 € (in stock for the time being)
80 seeds of SR for 5,60 € (in stock at the time being)
Ordered both, will probably split in half, send one half over the big pond for distribution and distribute the other here.
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Thanks Auxin for the important reference. Maybe this can be important in future.
I so knew I'm right on setting out to collect possibly ALL salviae !
The Salvia species are really very interesting. Many have pain suppressing properties. Especially when you make extracts from them. Recently, the ethanolic extract of aerial part of salvia aethiopis exhibited in tests moderate to weak activity towards delta and kappa opioid receptors.
Salvia guaranitica contains Cirsiliol which has soothing and hypnotic properties in pure form like benzodiazepines.
Other species are no less interesting. It comes a lot more in future.
I thought this study would be of interest to those researching de novo
analgesia in salvias, as well as shine some light on the vastly under-researched kappa opioid receptor network and how it plays with the rest of the brain.
http://www.sciencedirect.com/science/article/pii/S0091305705004004
http://www.sciencedirect.com/science/article/pii/S0006322307007366
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Go BC, making things happen! I'm not gonna ask for some, with so few and all I've got on my plate I wouldn't wanna risk failing with them :P
But once y'all establish them and get propagation figured out, I'd love to get one. Probably gonna wait until I can keep Sally D and Sally officinalis alive first (I can clone Mimosa, which is supposed to be hard, but I can't keep sages alive...)
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Since someone hmu on easy S G and S R seeds I have checked pricing, as suggested stuff is ridicolous.
Can have:
190 seeds of S G for 1,50 € (in stock for the time being)
80 seeds of SR for 5,60 € (in stock at the time being)
Ordered both, will probably split in half, send one half over the big pond for distribution and distribute the other here.
hy BC, that is a better price then i found.. Ordered from ebay glutinosa 4$30 and recognita4$20..
So if you want i would purchase/trade some from you, or goup buy, or something let me know, :)
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Salvia G arrived, at 10 seeds per request i can make about 80 members happy. Also taking double and tripple orders for those serious and wanting to avoid genetic bottlenecks.
The other Sally is in the making. I'll set up a giveaway and link here and back.
http://sharetheseeds.me/forum/index.php?topic=4855.0
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Salvia G arrived, at 10 seeds per request i can make about 80 members happy. Also taking double and tripple orders for those serious and wanting to avoid genetic bottlenecks.
The other Sally is in the making. I'll set up a giveaway and link here and back.
http://sharetheseeds.me/forum/index.php?topic=4855.0
I'll take a quadruple order, while you're at it 8)
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I'd be interested.
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To my way of thinking this is a good thing and there are 2 major ways of exploiting it
1. Grow massive quantities of the species and extract the salvinorin.
2. Using gene modification insert the genes for salvinorin into other plants or back into salvia plants which are hardy.
#1 will work if extraction is not that hard and if large amounts of biomass can be grown quickly and easily. You don't want to wait years for a few milligrams of salvinorin. Possibly graft it onto hardy rootstock? Hydroponics maybe?
#2 is not that far out. There are many labs doing gene splicing work on plants and animals. It would cost something to do it and there is no guarantee the sal production will be high and remain high. It may fade out after time or with lots of cloning
Sometimes genes inserted into a cell remain robust and are found in the offspring when the cell divides. But as in the case of bacteria which produce morphine, insulin or other drugs, the trait is rapidly lost and the bacteria must be replaced constantly. Since saliva produces it naturally, it may be that this would carry over well into clones or even seeds.
Some day we will be able to buy gene splicing kits for perhaps $1000 or less and do it ourselves. Making all sorts of drugs, thc, cbd, etc and etc. Since gene splicing to create drugs is an industry now, the technology is there, its just a matter of tapping into it. Anyone know a mad scientist who might be interested?
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Super interesting! If anyone wants a copy, you are welcome to pm me
Want a copy, pmed you.
Anyone know a mad scientist who might be interested?
phytochemist
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dang, are any of these still around, particularly the ones with salvinorin b? looking for savlia potentillifolia, but only found one site that doesn't ship to my country (US).
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but only found one site
Website name please.
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Thought I'd post a couple of my S. recognita seedlings that pulled through winter mostly unharmed if a little etiolated:
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Full text can be requested from the author here - https://www.researchgate.net/publication/318549211_Screening_of_Hallucinogenic_Compounds_and_Genomic_Characterisation_of_40_Anatolian_Salvia_Species_Hallucinogenic_Compounds_and_Genomic_Analysis_of_Salvia_Species
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...loving those seedlings by the way MG!
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The Salvia glutinosa(sp?) seeds I had gotten at some point last year were unfortunately crushed on arrival. Wish I coulda got them going. Only like two seeds were uncrushed but must have been damaged as they never sprouted. Better vibes next round.
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.
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do we know the 35 other salvia tested without potential?
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The species studied are the following:
adenocaulon
aucheri
multicaulis
tomentosa
adenophylla
heldreichiana
pisidica
blepharochlea
macrochlamys
trichoclada
suffruticosa
potentillifolia
bracteata
cryptantha
cadmica
recognita
huberi
rosifolia
hydrangea
kronenburgii
pachystachys
caespitosa
aethiopis
aramiensis
atropatana
candidissima
chionantha
frigida
microstegia
cilicica
limbata
syriaca
poculata
staminea
nemorosa
russellii
sclarea
virgata
verticillata
glutinosa
divinorum
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are tehre quantities available for each species by chance?